Fig. 2. Microarray analysis reveals that ALK inhibitors alter the expression of cell cycle-related genes.

a Summary of microarray analysis. NB39-nu cells were treated with 1000 nM of the two ALK inhibitors for 24 h, and were then subjected to a microarray analysis (see the Methods section). The numbers of genes upregulated, or downregulated, in common in NB39-nu cells treated with the two ALK inhibitors are shown as Venn diagrams. b Summary of Gene ontology (GO) analysis. A gene ontology analysis using Metascape (http://metascape.org/) was performed. The GO terms of the upregulated genes (left) and the downregulated genes (right) are shown. (See also supplementary Table 1). c,d Alterations in the expression of cell cycle-related genes in response to the two ALK inhibitors. NB39-nu or NB1 cells were treated with 1000 nM of the two ALK inhibitors for 24 h (c) or NB39-nu cells were treated as indicated (d) and qRT-PCR analysis (c) and an immunoblot analysis (d) were carried out. e The two ALK inhibitors induce dephosphorylation of pRb. NB39-nu cells were treated with either 1000 nM crizotinib (CR) or alectinib (AL) for the indicated times and subjected to immunoblot analysis. CLTC is the loading control. All quantitative data are shown as the mean ± SD (n = 3). *p < 0.05. All experiments were repeated at least three times