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. 2018 Jan 29;169(2):231–241. doi: 10.1007/s10549-018-4664-7

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© The Author(s) 2018

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

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Fig. 1 — Flowchart of patient cohort and biomarker expression in primary tumour, synchronous lymph node metastases and asynchronous recurrences. Due to limited remaining tissue material, synchronous lymph node metastasis and asynchronous recurrences were only evaluable in 135 and 39 patients, respectively. Boxes are inserted into the flowchart displaying information on matched pairs, i.e. numbers of primary tumours and nodes, and primary tumours and recurrences displaying identical scoring of each marker, respectively (positive–positive or negative–negative)