Fig. 7.

Phenotypes of tassels, spikelets and pollen grains in three knockout lines generated by a CRISPR/Cas9 system. Compared with the wild-type (a) sequence, deletions of 1, 192, and 453 bp were detected in the three knockout lines Ms33-Cas9-1 (b), Ms33-Cas9-2 (c), and Ms33-Cas9-3 (d), respectively. e1 Wild-type tassel, e2 spikelet and e3 mature pollen grains stained with 1% I2-KI solution. f2 The ms33-6029 mutant anthers were not exserted from f1 the tassel, and f3 no pollen grains were present in the anthers. g1–i1 The tassels, g2–i2 spikelets and g3–i3 absence of pollen grains in the three knockout lines (Ms33-Cas9-1 ~ 3). Bars = 1 mm (e2–i2), 150 μm (e3–i3)