Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2018 May 10;8:7450. doi: 10.1038/s41598-018-25816-8

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Cytotoxicity of an anti-nucleolin VHH-Fc antibody against nucleolin-overexpressing cancer cells. (a) An anti-nucleolin VHH-Fc antibody (αNCL-VHH-Fc) was generated by fusing αNCL-CDR3 to a human IgG1 Fc region and then incubated with (b) MDA-MB-435S or (c) 4T1 nucleolin-overexpressing cancer cells, at concentrations up to 1000 nM, for 72 h at 37 °C. The parental VHH-Fc antibody, without the targeting component to nucleolin, was included as control. In the end of the incubation, cytotoxicity was assessed by the MTT assay. Data represent the mean ± SD of three independent experiments, performed in duplicate. Differences in cytotoxicity between the VHH-Fc antibodies were evaluated by Student’s t-test (**p < 0.01, ***p < 0.001).