Figure 5.

Cytotoxicity of anti-nucleolin VHHs in the presence of PBCMs, against MDA-MB-435S cells. MDA-MB-435S cells, previously cultured in a RTCA plate for 24 h, were incubated with protein, without (solid lines) or with human PBMCs (at a target cells/effector cells ratio of 1:10 or 1:5, dotted lines), for 72 h at 37 °C. Cell index, normalized to the beginning of the incubation, was measured every 15 min, using the xCELLigence system. ADCC effect of the anti-nucleolin VHH-Fc antibody (red) was further supported upon assessing its activity versus (a) 25 nM parental VHH-Fc (blue), (b) 50 nM αNCL-CDR3 VHH (green), (c) 25 nM parental VHH-Fc (blue) versus 50 nM parental VHH (orange). Data are from a representative experiment, performed in duplicate.