Fig. 5. CARF knockdown attenuated cancer cell invasiveness and abolished EMT phenotype.

a Control and CARF siRNA transfected crystal violet-stained SKOV-3 cells showing reduced proliferation in latter with increasing siRNA (10–200 pM) concentrations. Immunostaining of CARF (with 50 pM siRNA) and cell viability (10–200 pM), respectively. b Invasion assay showing invaded cells and quantitation (below) in control and CARF knockdown cells. c Wound-healing assay (72 h) showing reduced cell migration in CARF knockdown cells; quantitation shown below. d Immunoblotting and e–g immunostaining of CARF, β-catenin (e), E-cadherin and vimentin (f), and fibronectin and N-cadherin (g), showing decrease in CARF knockdown cells; E-cadherin showed increase; quantitation of their fluorescence intensities are shown below