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. 2018 May 10;9(5):543. doi: 10.1038/s41419-018-0575-0

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — a, b Huh7 and HepG2 cells were treated with 30 μM corylin for 48 h, and the expression of RAD51-AS1 and RAD51 was analyzed by quantitative real-time RT-PCR. GAPDH served as an internal control. ***p < 0.001. c Huh7 cells were transfected with siRNA against lncRNA RAD51-AS1 (siRAD51-AS1) or non-targeting siRNA (si-CTR) at 50 nM, and then co-treated with corylin (30 μM). After 48-h treatment, cells were harvested and lysates were subjected to western blotting to detect the level of RAD51. β-Actin served as an internal control (left panel). The quantitative results are shown in the right panel. ***p < 0.001. d Huh7 and HepG2 cells were transfected with 1 μg pcDNA-RAD51-AS1 expressing plasmid or empty vector for 48 h. The protein level of RAD51 was analyzed by western blotting (left panel). The quantitative results are shown in the right panel. ***p < 0.001. e, f Real-time PCR analysis shows the mRNA levels of RAD51-AS1 and RAD51 during the above-mentioned two treatments. GAPDH served as an internal control. All data were expressed as the mean ± S.D. of three independent experiments. **p < 0.01, ***p < 0.001