Table 2.

GI JEV VLP-vaccinated pigs challenged with GI and GIII viruses.

Immunogens	JEV challenge	Viremiaa		Tonsilsb	Lymph nodes	Brain	Body temperaturec	Fold increase in FRμNT50d
		Viral titers (ffu/ml) (mean ± SD)	Viral RNA (copies/ml) (mean ± SD)	Viral RNA (copies/g) (mean ± SD)
PBS	YL2009-4	3.6 ± 0.1	6.3 ± 0.5	7.1 ± 0.3	6.1 ± 0.2	5.0 ± 0.4	40.7	1093.0*
PBS	YL2009-4	3.4 ± 0.1	5.6 ± 0.3	6.3 ± 1.2	4.5 ± 0.1	5.2 ± 0.4	40.8	938.0*
PBS	YL2009-4	4.6 ± 0.2	7.1 ± 0.2	6.9 ± 0.1	7.0 ± 0.2	5.8 ± 0.3	41.1	1966.6*
PBS	YL2009-4	4.7 ± 0.2	6.3 ± 0.3	7.2 ± 0.5	6.7 ± 0.3	5.1 ± 0.1	40.7	1698.2*
PBS	CH1392	2.8 ± 0.2	5.8 ± 0.2	5.6 ± 1.3	4.2 ± 0.1	4.5 ± 0.5	40.2	2048.0*
PBS	CH1392	2.5 ± 0.2	5.1 ± 0.1	6.1 ± 0.1	4.5 ± 0.1	4.6 ± 0.3	39.8	1220.6*
PBS	CH1392	2.5 ± 0.3	5.6 ± 0.4	6.2 ± 0.4	4.8 ± 0.2	5.1 ± 0.1	39.9	814.8*
PBS	CH1392	2.8 ± 0.2	5.8 ± 0.1	6.6 ± 0.2	5.2 ± 0.1	5.2 ± 0.2	39.5	1367.0*
GI VLP	YL2009-4	<1.3	<2.0	<2.0	<2.0	<2.0	38.3	0.8
GI VLP	YL2009-4	<1.3	<2.0	<2.0	<2.0	<2.0	38.4	3.6
GI VLP	YL2009-4	<1.3	<2.0	<2.0	<2.0	<2.0	38.1	1.6
GI VLP	YL2009-4	<1.3	<2.0	<2.0	<2.0	<2.0	38.5	9.3*
GI VLP	CH1392	<1.3	<2.0	<2.0	<2.0	<2.0	37.8	0.4
GI VLP	CH1392	<1.3	<2.0	<2.0	<2.0	<2.0	37.9	0.3
GI VLP	CH1392	<1.3	<2.0	<2.0	<2.0	<2.0	38.2	1.3
GI VLP	CH1392	<1.3	<2.0	<2.0	<2.0	<2.0	37.9	3.3
GI VLP	CH1392	<1.3	<2.0	<2.0	<2.0	<2.0	38.5	6.6*

^aThe viral titers and viral RNA in pig plasma was detected and duplicated by micro-plaque assay and real-time RT-PCR at 2 DPI. ^bThe viral RNA was quantified in duplicate samples of tonsils, lymph nodes, and brain from infected pig at 8 DPI by real-time RT-PCR. ^cThe highest body temperature of swine was indicated during 8 days post-challenge. ^dIn comparing FRμNT₅₀ at 8 DPI to the value before challenge, a fold increase was indicated and a ≥4-fold increase was noted by an asterisk.