Table 2.
Detection of QTL associated with resistance to MLN at early and late stages of disease infection, and their physical positions and genetic effects of the QTL in four F3 populations
| Trait | QTL namea | Chr | Position (cM) | LOD | PVE (%) | Add | Dom | PVE (%) | QTL confidence interval | Physical position (bp) | Fav parent | ||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Left marker | Right marker | Left | Right | ||||||||||
| CML543 × LaPostaSeqC7-F71 | |||||||||||||
| MLN-early | qMLN_03-01 | 3 | 0 | 3.80 | 3.96 | − 0.09 | 0.17 | 48.22 | S3_1326446 | S3_1337663 | 1326446 | 1337663 | LPS_F71 |
| qMLN_03-130 | 3 | 157 | 27.48 | 37.80 | 0.27 | 0.00 | PZA00413_20 | PZA02299_16 | 125192432 | 130082791 | CML543 | ||
| qMLN_06-04 | 6 | 5 | 5.12 | 5.61 | 0.08 | 0.02 | S6_3869709 | S6_4015237 | 3869709 | 4015237 | CML543 | ||
| MLN-late | qMLN_01-252 | 1 | 68 | 3.09 | 3.41 | − 0.08 | 0.00 | 48.23 | S1_251157170 | PZA02269_4 | 251157170 | 252721946 | LPS_F71 |
| qMLN_03-130 | 3 | 157 | 21.37 | 27.16 | 0.29 | − 0.03 | PZA00413_20 | PZA02299_16 | 125192432 | 130082791 | CML543 | ||
| qMLN_06-19 | 6 | 46 | 8.52 | 9.40 | 0.14 | − 0.06 | S6_18924381 | S6_21021616 | 18924381 | 21021616 | CML543 | ||
| qMLN_08-174 | 8 | 222 | 5.06 | 5.45 | − 0.10 | 0.03 | S8_174216280 | S8_174222902 | 174216280 | 174222902 | LPS_F71 | ||
| CML444 × CML543 | |||||||||||||
| MLN-early | qMLN_01-246 | 1 | 304 | 4.57 | 4.99 | 0.07 | − 0.02 | 56.75 | S1_246489667 | S1_246540548 | 246489667 | 246540548 | CML543 |
| qMLN_03-146 | 3 | 123 | 30.07 | 43.84 | 0.25 | 0.01 | S3_146251234 | S3_146250249 | 146251234 | 146250249 | CML543 | ||
| qMLN_06-85 | 6 | 197 | 5.73 | 6.12 | 0.08 | 0.01 | S6_85416016 | S6_96909472 | 85416016 | 96909472 | CML543 | ||
| qMLN_08-123 | 8 | 94 | 3.81 | 4.23 | 0.02 | − 0.11 | S8_123055373 | S8_123469828 | 123055373 | 123469828 | CML543 | ||
| qMLN_10-81 | 10 | 213 | 4.22 | 4.43 | − 0.07 | 0.00 | S10_81827920 | S10_81829237 | 81827920 | 81829237 | CML444 | ||
| MLN-late | qMLN_01-241 | 1 | 282 | 7.02 | 10.46 | 0.00 | − 0.24 | 58.61 | S1_237487786 | S1_241184216 | 237487786 | 241184216 | CML543 |
| qMLN_03-146 | 3 | 124 | 20.08 | 24.26 | 0.26 | − 0.03 | S3_146250249 | S3_146251923 | 146250249 | 146251923 | CML543 | ||
| qMLN_05-190 | 5 | 251 | 9.55 | 10.44 | − 0.16 | 0.01 | S5_190677275 | PZA00352_23 | 190677275 | 191075557 | CML444 | ||
| qMLN_05-199 | 5 | 349 | 15.36 | 17.74 | 0.19 | 0.00 | S5_199499548 | S5_199499538 | 199499548 | 199499538 | CML543 | ||
| qMLN_05-207 | 5 | 432 | 4.06 | 4.25 | − 0.04 | 0.13 | S5_206890892 | PZA02015_11 | 206890892 | 207464707 | CML444 | ||
| qMLN_06-85 | 6 | 176 | 10.32 | 10.90 | 0.15 | 0.06 | S6_85203511 | S6_85206463 | 85203511 | 85206463 | CML543 | ||
| CML539 × CML444 | |||||||||||||
| MLN-early | qMLN_01-148 | 1 | 72 | 3.24 | 4.84 | − 0.07 | 0.01 | 42.15 | ZM00148950 | ZM00145696 | 148500000 | 160300000 | CML539 |
| qMLN_03-27 | 3 | 69 | 4.17 | 6.91 | 0.02 | − 0.11 | S3_27365043 | S3_29611811 | 27365043 | 29611811 | CML444 | ||
| qMLN_03-126 | 3 | 350 | 6.32 | 10.55 | 0.00 | 0.14 | S3_122493752 | S3_126171099 | 122493752 | 126171099 | CML444 | ||
| qMLN_04-117 | 4 | 59 | 5.54 | 9.13 | 0.09 | − 0.04 | ZM00148564 | ZM00146885 | 106500000 | 117900000 | CML444 | ||
| qMLN_05-131 | 5 | 150 | 4.33 | 6.46 | 0.08 | 0.02 | ZM00147053 | ZM00148930 | 115900000 | 131900000 | CML444 | ||
| qMLN_06-03 | 6 | 185 | 5.42 | 8.96 | − 0.05 | − 0.11 | S6_2385933 | S6_3039514 | 2385933 | 3039514 | CML539 | ||
| qMLN_09-100 | 9 | 66 | 4.37 | 7.49 | − 0.08 | − 0.02 | S9_99440296 | S9_100608734 | 99440296 | 100608734 | CML539 | ||
| qMLN_10-71 | 10 | 32 | 5.42 | 8.21 | 0.09 | 0.01 | S10_71178058 | S10_71665925 | 71178058 | 71665925 | CML444 | ||
| MLN-late | qMLN_03-159 | 3 | 487 | 5.48 | 9.13 | − 0.10 | 0.03 | 36.95 | S3_159801859 | S3_158524074 | 159801859 | 158524074 | CML539 |
| qMLN_05-171 | 5 | 167 | 3.79 | 6.16 | 0.09 | − 0.03 | ZM00147844 | ZM00150817 | 154700000 | 171900000 | CML444 | ||
| qMLN_06-06 | 6 | 202 | 8.15 | 14.15 | − 0.13 | − 0.01 | S6_5159730 | S6_6270908 | 5159730 | 6270908 | CML539 | ||
| qMLN_06-16 | 6 | 215 | 5.68 | 9.51 | 0.00 | − 0.16 | ZM00150684 | S6_16291106 | 12700000 | 16291106 | CML444 | ||
| qMLN_09-100 | 9 | 230 | 3.51 | 7.27 | 0.01 | − 0.13 | ZM00149322 | ZM00145937 | 96500000 | 116800000 | CML444 | ||
| Mo37 × CML144 | |||||||||||||
| MLN-early | qMLN_03-130 | 3 | 129.6 | 5.6 | 16.63 | 0.11 | 0.03 | 38.76 | PHM1745_16 | PZA00920_1 | 129095914 | 131969810 | CML144 |
| qMLN_09-100 | 9 | 112.5 | 5.33 | 16.32 | − 0.05 | − 0.43 | PZA00060_2 | PZB00221_3 | 95769540 | 113201792 | Mo37 | ||
| MLN-late | qMLN_03-129 | 3 | 128.6 | 5.58 | 13.97 | 0.18 | − 0.04 | 39.13 | PZA00413_20 | PHM1745_16 | 113820730 | 129095914 | CML144 |
| qMLN_06-19 | 6 | 14.2 | 3.18 | 9.61 | − 0.14 | − 0.02 | PZA00158_2 | PZA00440_1 | 13254840 | 21599925 | Mo37 | ||
| qMLN_08-157 | 8 | 156.4 | 4.32 | 13.94 | − 0.15 | 0.12 | PZA00460_3 | PHM4786_9 | 156320002 | 157402090 | Mo37 | ||
MLN-early indicates MLN score 21 days after first post inoculation; MLN-late indicates MLN score 42 days after first post inoculation; fav parent indicates parental genotype from where favorable allele for MLN resistance is contributing. Markers with italicized letters are the QTL consistent across MLN-early and MLN-late
LOD logarithm of odds, Add additive effect, Dom dominance effect, PVE phenotypic variance explained
aQTL name composed by the trait code followed by the chromosome number in which the QTL was mapped and a physical position of the QTL