FIGURE 3.

ROS metabolism of the P. anserina WT is affected by quercetin. (A) Qualitative determination of superoxide anion release by a histochemical NBT staining and hydrogen peroxide release by a histochemical DAB staining in P. anserina WT cultures (n = 4) treated with quercetin (Quer) or DMSO (Con). (B) Quantitative measurement of H₂O₂ release from P. anserina WT cultures treated with quercetin (Quer; n = 5) compared with DMSO (Con; n = 5) treatment. The mean release of the DMSO-treated WT was set to 1. Error bars correspond to the standard deviation and P-value was determined by two-tailed Student’s t-test. ^∗∗P < 0.01. (C) Representative “in-gel” superoxide dismutase (SOD) (PaSOD1 and PaSOD2) activity staining from total protein extract of P. anserina WT cultures treated with quercetin (Quer; n = 4) or DMSO (Con; n = 4). (D) Representative “in-gel” SOD (PaSOD3) activity staining from mitochondrial protein extract of P. anserina WT cultures treated with quercetin (Quer; n = 3) or DMSO (Con; n = 3). (E) Representative “in-gel” peroxidase and (F) catalase activity staining from total protein extracts of P. anserina WT cultures treated with quercetin (Quer; n = 4) or DMSO (Con; n = 4).