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. 2018 May 4;9:160. doi: 10.3389/fgene.2018.00160

FIGURE 5.

FIGURE 5

Lifespan extension by quercetin is dependent of O-methyltransferase activity. (A) Lifespan of ΔPaMth1 cultures grown on standard M2 medium containing DMSO (Con; n = 34) or quercetin (Quer; n = 31; P = 0.13878). (B) Relative mean lifespan of cultures from (A). Data shown as relative values compared to the DMSO control, which was set to 100%, ±standard derivation. (C) Relative mean growth rate of cultures from (A). Data shown as relative values compared to the DMSO control, which was set to 100%, ±standard derivation. (D) Lifespan of P. anserina Mth1_OEx cultures grown on standard M2 medium containing DMSO (Con; n = 25) or quercetin (Quer; n = 25; P = 0.00012). (E) Relative mean lifespan of cultures from (D). Data are shown as relative values compared to the DMSO control, which was set to 100%, ±standard derivation. (F) Relative mean growth rate of cultures from (D). Data are indicated as relative values compared to the DMSO control, which was set to 100%, ±standard derivation. (G) Representative western blot analysis of total protein extract of P. anserina WT (n = 4) and PaMth1_OEx cultures (OEx; n = 4) treated with DMSO (Con) or quercetin (Quer). (H) Quantification of PaMTH1 protein level normalized to the Coomassie-stained gel. Protein level in WT cultures treated with DMSO was set to 1. Error bars correspond to the standard deviation and P-values were determined by two-tailed Student’s t-test. ∗P < 0.05; ∗∗P < 0.01, ∗∗∗P < 0.001.