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. 2018 May 4;9:398. doi: 10.3389/fphys.2018.00398

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Copyright © 2018 Raisch, Yanoff, Larsen, Farooqui, King, Veeraraghavan, Gourdie, Baker, Arnold, AlMahameed and Poelzing.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Absolute, but not relative, perinexal widths differ between observers. (A) Representative TEM image from human left atrial appendage measured by two different observers. Arrows indicate different starting and ending points between observers. (B) Perinexal width (Wp) from (A) are different between observers 1 and 2. (C) Wp correlates between observers using data from the first 20 patients (149 images) but the y-intercept is different. (D) Wp changes from the gap junction edge up to 30 nm (^∗p < 0.05). (E) Observers collect similar numbers of Wp measurements up to 105 nm from gap junction edge. (^∗p < 0.05 relative to numbers at previous distance).