Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2018 May 10;9(5):539. doi: 10.1038/s41419-018-0546-5

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 2 — a Following 5 h infection, with or without Shield1 (Sh1) pretreatment, IAV RNA expression levels were determined using RT and qPCR primer/probe sets specific for the NP, NS1, PB1, M1 and M2 genes. b–d ATG5^DD cells, with or without Sh1 treatment, were infected with IAV for 16 h at the indicated MOI. The gating strategy after nucleoprotein (NP) immunostaining and flow cytometry is shown for two samples: uninfected, and infected at an MOI of 7 (b). The percentage of NP-expressing cells (c) and the geometric mean fluorescent intensity (GMFI) of NP per cell (d) were determined by flow cytometry. e, f IAV M2 expression was determined using flow cytometry (e) and immunoblotting (f) following 16 or 20 h infection at the indicated MOI, respectively. g ATG5^DD cells, pretreated or not with Shield1 (Sh1), were infected for 16 h at the indicated MOI. NP RNA expression in supernatants was analysed by RT–qPCR. h ATG5^DD cells, pretreated or not with Shield1 (Sh1), were infected at MOI 3 for the indicated times; haemagglutination assays were used to quantify of the number of haemagglutinin units (HAUs) per millilitre of supernatant. i Atg5^+/+ or Atg5^–/– were infected with IAV at the indicated MOI for 16 h. Percentages of NP-expressing cells was determined by flow cytometry. Graphs show mean and standard deviation of biological triplicates, and data are representative of two experiments. ns, not significant (one-tailed unpaired t-test followed by Holm’s multiple testing correction)