Fig. 3.

LbCpf1 targeted to Vegfa or Hif1a reduces the area of laser-induced CNV in mice. a–c At day 42 post-injection of AAV-Cpf1, mice were treated with laser to induce CNV. Aflibercept (2.5 μg) was intravitreally injected immediately after laser treatment. No AAV indicates no injection of AAV or aflibercept as a control. Three days after laser treatment, Vegfa protein levels were measured by ELISA in the retina and RPE complex. One week after laser treatment, the CNV area was analyzed. a Representative laser-induced CNV stained with isolectin B4 in mouse eyes injected with AAV-Cpf1 targeted to DNMT, Vegfa, or Hif1a. Scale bar is 200 μm. b The CNV area. Error bars indicate s.e.m. (n = 20). One-way ANOVA and Tukey’s post-hoc tests, * P < 0.05, ** P < 0.01, *** P < 0.001, ns, not significant. c VEGFA protein levels. Error bars indicate s.e.m. (n = 10). One-way ANOVA and Tukey’s post-hoc tests. *, P < 0.05; **, P < 0.01; ns not significant. d–e Six weeks after intravitreal injection of PBS or aflibercept (2.5 μg), mice were treated with laser to induce CNV. One week after laser treatment, the CNV area was analyzed. d Representative laser-induced CNV stained with isolectin B4 in mouse eyes injected with PBS or aflibercept. Scale bar is 200 μm. e The CNV area. Error bars indicate s.e.m. (n = 30). Student’s t-test. ns, not significant