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Bacterial challenge assay with phage vB_EcoS-B2 to E. coli MG1655. vB_EcoS-B2 was added at an MOI of 10 to the bacterial culture after 1.5 h incubation (OD600 = 0.25) (close circles). OD600 was recorded at 15 min intervals, over a period of 300 min. A bacterial culture inoculated with SM buffer instead of vB_EcoS-B2 was used as the negative control (open circles). Each value is the average from three different cultures.
