Table 2.
Predicted ORFs and genes encoded by the vB_EcoS-B2 genome.
| ORFs | Start | Stop | Directions | No. of residues | MW(da) | pI | Predicted molecular function |
|---|---|---|---|---|---|---|---|
| ORF1 | 1 | 2,292 | + | 763 | 86167.7 | 8.56 | DNA polymerase I |
| ORF2 | 2,292 | 2,570 | + | 92 | 10147.2 | 10.39 | Hypothetical protein |
| ORF3 | 2,611 | 2,784 | + | 57 | 6825.7 | 10.35 | Hypothetical protein |
| ORF4 | 2,822 | 3,046 | + | 74 | 8761.5 | 9.94 | Hypothetical protein |
| ORF5 | 3,051 | 4,475 | + | 474 | 53461.8 | 9.55 | DNA helicase |
| ORF6 | 4,472 | 5,167 | + | 231 | 26496.7 | 8.27 | Putative DNA cytosine methyltransferase C5 |
| ORF7 | 5,164 | 5,487 | + | 107 | 11695 | 9.14 | Putative HNH endonuclease |
| ORF8 | 5,569 | 6,033 | + | 154 | 17882.3 | 8.19 | Hypothetical protein |
| ORF9 | 6,020 | 6,505 | + | 161 | 18708.4 | 9.72 | DNA methyltransferase |
| ORF10 | 6,486 | 6,710 | + | 74 | 8686.6 | 8.9 | Hypothetical protein |
| ORF11 | 6,707 | 7,177 | + | 156 | 17872.8 | 6.25 | Hypothetical protein |
| ORF12 | 7,242 | 7,796 | + | 184 | 20488.5 | 4.93 | Hypothetical protein |
| ORF13 | 7,839 | 8,414 | + | 191 | 21491.9 | 6.78 | Hypothetical protein |
| ORF14 | 8,442 | 10,265 | – | 607 | 65194.4 | 4.79 | Putative tail tip protein |
| ORF15 | 10,346 | 10,630 | + | 94 | 10251.1 | 7.28 | Hypothetical protein |
| ORF16 | 10,582 | 11,253 | – | 223 | 23211.6 | 8.18 | Hypothetical protein |
| ORF17 | 11,256 | 11558 | – | 100 | 10216.6 | 8.5 | Hypothetical protein |
| ORF18 | 11,596 | 15,015 | – | 1,139 | 125892.9 | 5.11 | Putative tail fiber protein |
| ORF19 | 15,012 | 15629 | – | 205 | 21245.4 | 8.71 | Putative tail assembly protein I |
| ORF20 | 15,620 | 16,360 | – | 246 | 27577.5 | 4.95 | Tail assembly protein |
| ORF21 | 16,363 | 17,151 | – | 262 | 28806.3 | 4.88 | Putative minor tail protein L |
| ORF22 | 17,148 | 17,747 | – | 199 | 21696.3 | 6.52 | Putative minor tail protein |
| ORF23 | 17,784 | 20,426 | – | 880 | 93307.2 | 9.93 | Putative tail length tape-measure protein 1 |
| ORF24 | 20,426 | 20,518 | – | 30 | 3341.4 | 10.5 | Hypothetical protein |
| ORF25 | 20,490 | 20,681 | – | 63 | 6931.5 | 8.51 | Hypothetical protein |
| ORF26 | 20,821 | 21,051 | – | 76 | 8543.3 | 5.77 | Hypothetical protein |
| ORF27 | 21,135 | 21,497 | – | 120 | 13958.5 | 4.78 | Hypothetical protein |
| ORF28 | 21,567 | 22,292 | – | 241 | 25755.3 | 5.82 | Putative major tail protein |
| ORF29 | 22,354 | 22,776 | – | 140 | 15087.1 | 4.73 | Hypothetical protein |
| ORF30 | 22,776 | 23,369 | – | 197 | 21918 | 11.19 | Putative tail protein |
| ORF31 | 23,371 | 23,730 | – | 119 | 13053.8 | 9.59 | Putative structural protein |
| ORF32 | 23,711 | 23,827 | – | 38 | 3990.7 | 3.76 | Hypothetical protein |
| ORF33 | 23,829 | 24,359 | – | 176 | 18924.7 | 3.89 | Hypothetical protein |
| ORF34 | 24,388 | 25,488 | – | 366 | 38395.4 | 7.66 | Major head protein |
| ORF35 | 25,586 | 26,287 | – | 233 | 25384.1 | 5.51 | Hypothetical protein |
| ORF36 | 26,388 | 26,756 | + | 122 | 13128.6 | 5.65 | Hypothetical protein |
| ORF37 | 26,796 | 27,560 | + | 254 | 28937.1 | 8.29 | Hypothetical protein |
| ORF38 | 27,574 | 27,732 | – | 52 | 5739.7 | 4.33 | Hypothetical protein |
| ORF39 | 27,686 | 28,015 | – | 109 | 11931.4 | 9.88 | Hypothetical protein |
| ORF40 | 28,008 | 29,111 | – | 367 | 40988.6 | 9.94 | Head morphogenesis protein |
| ORF41 | 29,095 | 30,615 | – | 506 | 55192.3 | 4.51 | Phage structural protein |
| ORF42 | 30,627 | 32,012 | – | 461 | 52196.7 | 7.52 | Terminase large subunit |
| ORF43 | 32,012 | 32,584 | – | 190 | 21084 | 8.05 | Putative terminase small subunit |
| ORF44 | 32,694 | 33,836 | – | 380 | 42737.4 | 7.35 | Putative phosphoesterase |
| ORF45 | 33,857 | 34,057 | + | 66 | 7221.1 | 8.22 | Hypothetical protein |
| ORF46 | 34,074 | 34,241 | – | 55 | 5603.2 | 4.48 | Hypothetical protein |
| ORF47 | 34,258 | 34,749 | – | 163 | 18156.3 | 9.41 | Lysozyme |
| ORF48 | 34,736 | 34,981 | – | 81 | 8870 | 8.51 | Putative holin-like class I protein |
| ORF49 | 34,978 | 35,268 | – | 96 | 10183.1 | 8.23 | Putative holin-like class II protein |
| ORF50 | 35,323 | 35,622 | – | 99 | 11796 | 9.72 | Hypothetical protein |
| ORF51 | 35,687 | 35,833 | – | 48 | 5626.5 | 11.71 | Hypothetical protein |
| ORF52 | 35,830 | 36,363 | – | 177 | 20721.2 | 10.59 | Hypothetical protein |
| ORF53 | 36,360 | 36,494 | – | 44 | 5107.7 | 3.43 | Hypothetical protein |
| ORF54 | 36,485 | 36,670 | – | 61 | 7413.4 | 11.48 | Hypothetical protein |
| ORF55 | 36,770 | 37,075 | – | 101 | 11091.8 | 10.31 | Hypothetical protein |
| ORF56 | 37,072 | 37,206 | – | 44 | 5206.2 | 8.24 | Hypothetical protein |
| ORF57 | 37,297 | 37,554 | – | 85 | 9233.9 | 11.49 | Hypothetical protein |
| ORF58 | 37,609 | 39,861 | – | 750 | 82443.1 | 6.15 | Hypothetical protein |
| ORF59 | 39,872 | 40,201 | – | 109 | 12545.1 | 8.47 | DNA recombination nuclease inhibitor gamma |
| ORF60 | 40,424 | 41,032 | + | 202 | 23053.6 | 4.52 | Hypothetical protein |
| ORF61 | 41,081 | 41,332 | + | 83 | 9953.6 | 9.91 | Hypothetical protein |
| ORF62 | 41,333 | 41,521 | + | 62 | 7168 | 8.22 | Hypothetical protein |
| ORF63 | 41,521 | 42,951 | + | 476 | 52319.8 | 8.73 | Hypothetical protein |
| ORF64 | 42,944 | 43,186 | + | 80 | 9323.4 | 6.49 | Hypothetical protein |
| ORF65 | 43,277 | 44,059 | + | 260 | 29909.5 | 4.55 | Hypothetical protein |