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. 2018 May 4;9:593. doi: 10.3389/fpls.2018.00593

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Copyright © 2018 Wang, Zhang, Li, Li, Bi, Crawford and Wang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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FIP1 affects nitrate uptake and accumulation. Seedlings were grown in ½ MS solution for 7 days and their nitrate content was quantified. (A) The nitrate concentration in the seedlings. (B) The nitrate concentration remaining in the solution after 7 days of seedlings growth. (C) Nitrate uptake in seedlings grown for 7 days in 2.5 mM ammonium succinate and then treated with 5 mM KNO₃ for the indicated periods. The nitrate concentration in the roots (D) and shoots (E). Error bars represent the SD of the biological replicates (n = 5). Asterisks indicate significant differences in the transgenic lines in comparison with the WT (p < 0.05, U-test). FW, fresh weight.