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. 2018 May 4;9:593. doi: 10.3389/fpls.2018.00593

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Copyright © 2018 Wang, Zhang, Li, Li, Bi, Crawford and Wang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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FIP1 regulates the expression of genes involved in nitrate transport and assimilation in the roots and shoots of seedlings grown for 7 days in ½ MS solution. (A,B) The relative expression levels of nitrate transport genes NRT1.5 and NRT1.8 were determined in the roots (A) and shoots (B) using qPCR. (B,C) The expression levels of the nitrate assimilation genes NIA1 and NiR were detected in the roots (C) and shoots (D) using qPCR. (E) Nitrate reductase (NR) activity and (F) amino acid content in fip1 roots and shoots. Error bars represent the SD of the biological replicates (n = 5). Asterisks indicate significant differences in the transgenic lines in comparison with the WT (p < 0.05, U-test).