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FIP1 can affect the APA in 3′-UTR of NRT1.1 mRNA. Polyacrylamide gel electrophoresis (PAGE) was run to separate the amplified bands of different 3′-UTR polyadenylated forms of NRT1.1 in WT, fip1, cpsf30, and fip1cpsf30. The seedlings were grown on 10 mM KNO3 for 7 days and total RNA was extracted for RT-PCR.
