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Summary of enzyme purification
| Purification Step | Total Protein | Specific Activity | Yield | Purification |
|---|---|---|---|---|
| mg | pkat mg−1 | % | -fold | |
| Crude extract | 523 | 9.98 | 100 | 1 |
| Butyl Toyopearl | 160 | 34.3 | 105 | 3.44 |
| Hydroxylapatite | 33.8 | 254 | 164 | 25.5 |
| DEAE Sephacel | 7.09 | 261 | 35.3 | 26.2 |
| NA-Sepharose | 0.84 | 1298 | 20.8 | 130 |
