Table 4.
Inhibition of mycelial growth, spore germination and germ tube elongation of Lasiodiplodia theobromae by the crude culture filtrate of the three BCA candidates either obtained from fish meal extract broth or colloidal chitin broth.
| BCA | Culture filtrate (%) | Colony diameter (mm) | Mycelial dry weight (g) | Conidia germination (%) | Germ tube length (μm) |
|---|---|---|---|---|---|
| (A) FISH MEAL EXTRACT BROTH | |||||
| BCA1 | 0 | 99.60 ± 0.22a | 81.05 ± 2.76a | 90.06 ± 1.77a | 59.86 ± 0.74a |
| 10 | 37.76 ± 1.39b | 28.40 ± 2.86b | 34.25 ± 1.74b | 47.24 ± 0.92b | |
| 25 | 18.87 ± 0.95c | 9.86 ± 1.08c | 21.31 ± 1.34c | 29.35 ± 0.94c | |
| 50 | 9.72 ± 0.84d | 3.88 ± 0.76d | 8.96 ± 0.36d | 18.59 ± 1.58d | |
| 75 | 0.00 ± 0.00e | 0.40 ± 0.10d | 3.19 ± 0.32d | 11.81 ± 0.84e | |
| 100 | 0.00 ± 0.00e | 0.00 ± 0.00d | 0.54 ± 0.09d | 3.50 ± 0.61f | |
| BCA2 | 0 | 99.51 ± 0.12a | 78.60 ± 3.60a | 89.17 ± 1.91a | 53.26 ± 1.53a |
| 10 | 39.72 ± 1.83b | 35.87 ± 2.08b | 40.36 ± 3.03b | 36.09 ± 1.91b | |
| 25 | 21.57 ± 0.89c | 15.92 ± 2.35c | 26.12 ± 1.41c | 28.17 ± 1.79c | |
| 50 | 11.33 ± 1.21d | 7.82 ± 1.15d | 16.19 ± 0.91d | 16.36 ± 0.01d | |
| 75 | 0.00 ± 0.00e | 3.15 ± 0.59de | 6.68 ± 0.37e | 6.32 ± 1.27e | |
| 100 | 0.00 ± 0.00e | 0.15 ± 0.08e | 0.97 ± 0.02f | 0.42 ± 0.14f | |
| (B) COLLOIDAL CHITIN BROTH | |||||
| BCA1 | 0 | 98.70 ± 0.68a | 80.39 ± 2.97a | 93.10 ± 2.13a | 60.69 ± 1.77a |
| 10 | 48.13 ± 2.05b | 40.49 ± 1.66b | 49.49 ± 2.86b | 36.58 ± 1.85b | |
| 25 | 26.94 ± 1.14c | 22.80 ± 1.82c | 28.55 ± 0.90c | 25.11 ± 1.28c | |
| 50 | 16.25 ± 1.23d | 13.58 ± 1.03d | 18.87 ± 0.93d | 13.21 ± 1.29d | |
| 75 | 0.00 ± 0.00e | 4.24 ± 0.92e | 8.48 ± 0.75e | 3.41 ± 0.51e | |
| 100 | 0.00 ± 0.00e | 0.48 ± 0.16e | 2.19 ± 0.42f | 0.00 ± 0.00e | |
| BCA3 | 0 | 99.84 ± 0.12a | 83.27 ± 2.64a | 93.32 ± 1.47a | 66.34 ± 1.81a |
| 10 | 29.27 ± 1.45b | 30.84 ± 2.79b | 28.04 ± 0.86b | 42.55 ± 1.49b | |
| 25 | 16.79 ± 1.05c | 13.12 ± 1.34c | 18.42 ± 1.03c | 17.44 ± 1.30c | |
| 50 | 6.85 ± 1.00d | 4.96 ± 0.95d | 10.03 ± 0.81d | 6.07 ± 0.83d | |
| 75 | 0.00 ± 0.00e | 0.00 ± 0.00e | 2.49 ± 0.45e | 0.00 ± 0.00e | |
| 100 | 0.00 ± 0.00e | 0.00 ± 0.00e | 0.00 ± 0.00e | 0.00 ± 0.00e | |
Values are means of three replicates ± SE. Values with the same letter within a column for each BCA are not significantly (P > 0.05) different, according to Duncan's multiple range test.
BCA1, isolate #12; Streptomyces samsunensis UAE1; BCA2, isolate #29; S. cavourensis UAE1; BCA3, isolate #44; Micromonospora tulbaghiae UAE1.