Fig. 2.

Confocal images of Psuc–Met/Ac-fixed cells. (A) A U2OS and a MCF10A cell with endogenous cytosolic γ-tubulin-containing structures (γ-tubules). The inset (white box) in the image to the left indicates the area shown in the image to the right, which illustrates γ-tubules at a higher magnification. (A–C) Cells were immunostained with an anti-γ-tubulin antibody produced in rabbit (RγTubulinAb, T3320) or mouse (MγTubulinAb, T6557) and co-stained with an anti-pericentrin (B) or an anti-α-tubulin antibody (C), as indicated. Scale bar: 10 μm. (B) The white arrowhead shows the centrosomes. (D) Graph showing the percentage of fixed cells with γ-tubules after fixation 3, 5 and 10 min with Psuc followed by Met/Ac fixation for 3, 5 and 10 min. A minimum of 100 cells was counted in each sample, and the percentage of cells with γ-tubules was calculated (mean ± SD; N = 5, *** P < 0.001).