Table 2.
Primer sequences of the target and reference genes.
| Gene | Primer sequence (5′ to 3′) | Product, bp | Gene Bank accession No. |
|---|---|---|---|
| β-actin | F: CGGGACATCAAGGAGAAGC | 273 | DQ845171 |
| R: ACAGCACCGTGTTGGCGTAGAG | |||
| IL-1β | F: TCCACCGCAAATGCTTCTAG | 132 | NM_214055.1 |
| R: TGCTGTCACCTTCACCGTTC | |||
| IFN-γ | F:GCTCTGGGAAACTGAGTGAC | 99 | JF906510 |
| R: TTTCGGTAGTCACTTGAGTA | |||
| Claudin-1 | F:GATTTACTCCTACGCTGGTGAC | 199 | AJ318102 |
| R: CACAAAGATGGCTATTAGTCCC | |||
| Occludin | F: GTAGTCGGGTTCGTTTCC | 167 | NM_001163647.2 |
| R: GACCTGATTGCCTAGAGTGT | |||
| ZO-1 | F:CTCTTGGCTTGCTATTCG | 256 | XM_003353439.2 |
| R: AGTCTTCCCTGCTCTTGC | |||
| CFTR | F: TTCCTCGTAGTCCTCGCC | 162 | AY585334.1 |
| R: GGTCAGTTTCAGTTCCGTTTG |
IL-1β = interleukin-1β; IFN-γ = interferon-γ; ZO-1 = zonula occludens-1; CFTR = cystic fibrosis transmembrane conductance regulators.