Table 4.

Formulation of reaction mixture for PCR amplification of 16-23S rDNA (from Mikkelsen et al., 2003).

Composition	Concentration	Volume, µL
Master mixture
Deoxynucleoside triphosphate (dNTP)	2.0 nmol/µL	5.0
Taq DNA polymerase	5.5 U/µL	0.2
MgCI2	25 nM	6.0
PCR buffer	10×	5.0
Forward primer – Lb16a	5 pmol/µL	2.0
Reverse primer – 23-1B	5 pmol/µL	2.0
PCR grade water	–	27.8
Total volume of master mixture for each sample		48
DNA crude extracts		2.0
Total reaction mixture for each sample		50