Table 1.

Composition of experimental diets (as-fed basis).¹

Item	Phase 1	Phase 2				Phase 3
	No AF	No AF		AF		No AF		AF
	No YC	No YC	YC	No YC	YC	No YC	YC	No YC	YC
Ingredient, %
Yellow corn	42.1	53.8	53.6	52.8	52.6	65.42	65.22	64.42	64.22
Soybean meal	25	30	30	30	30	30	30	30	30
DairyLac 80	20	9	9	9	9
Blood plasma	8	2.5	2.5	2.5	2.5
L-Lys HCl	0.05	0.15	0.15	0.15	0.15	0.1	0.1	0.1	0.1
DL-Met	0.05	0.05	0.05	0.05	0.05
Salt	2	0.22	0.22	0.22	0.22	0.3	0.3	0.3	0.3
Vitamin premix2	0.22	0.03	0.03	0.03	0.03	0.15	0.15	0.15	0.15
Mineral premix3	0.03	0.15	0.15	0.15	0.15	0.03	0.03	0.03	0.03
Dicalcium P	0.15	1.4	1.4	1.4	1.4	1.4	1.4	1.4	1.4
Limeston, ground	1.8	0.7	0.7	0.7	0.7	0.6	0.6	0.6	0.6
poultry fat	0.6	2	2	2	2	2	2	2	2
YC			0.2		0.2		0.2		0.2
Corn AF4				1	1			1	1
Calculated composition
DM, %	91.3	90.5	90.5	90.5	90.5	89.8	89.8	89.8	89.8
ME, Mcal/kg	3.45	3.42	3.42	3.42	3.42	3.42	3.42	3.42	3.42
CP, %	22.4	21.1	21.1	21.1	21.1	19.7	19.7	19.7	19.7
Lys, %	1.45	1.34	1.34	1.34	1.34	1.15	1.15	1.15	1.15
Cys + Met, %	0.84	0.76	0.76	0.76	0.76	0.66	0.66	0.66	0.66
Trp, %	0.3	0.26	0.26	0.26	0.26	0.23	0.23	0.23	0.23
Thr, %	0.98	0.83	0.83	0.83	0.83	0.74	0.74	0.74	0.74
Lactose sugar	16	7.2	7.2	7.2	7.2
Ca, %	0.91	0.8	0.8	0.8	0.8	0.72	0.72	0.72	0.72
Available P, %	0.56	0.4	0.4	0.4	0.4	0.33	0.33	0.33	0.33
Total P, %	0.89	0.72	0.72	0.72	0.72	0.64	0.64	0.64	0.64
AF, µg/kg				20	20			20	20
YC, %			0.2		0.2		0.2		0.2
Analyzed composition, %
DM	92.7	91.6	91.4	91.6	91.8	90.5	91.1	90.4	90.5
CP	21.5	20.2	19.6	21.8	19.5	19	20.3	18.6	18.6
ADF	3.15	2.75	2.21	3.48	2.92	2.91	3.35	3.7	3.47
Ca	0.79	0.74	0.83	0.66	0.83	0.73	0.69	0.66	0.69
P	0.86	0.68	0.74	0.67	0.73	0.64	0.64	0.64	0.63
AF5, µg/kg				27	39			36	29

AF = aflatoxin; YC = yeast cell wall based feed additive; ADF = acid detergent fiber.

¹Factor AF with the presence of 20 µg AF/kg. Factor YC with the supplementation of 2 g Integral A+/kg (Integral A + was a product of Alltech, Lexington, KY).

²Vitamin premix provided the following per kilogram of complete diet: 22,045,000 IU of vitamin A; 3,306,900 IU of vitamin D₃; 66,138 IU of vitamin K; 88 mg of vitamin B₁₂; 15,432 mg of riboflavin; 88,184 mg of niacin; 61,729 mg of d-pantothenic acid; 8,818 mg of menadione; 220 mg of biotin.

³Mineral premix provided the following composition: 1.10% of Cu; 198.0 mg/kg of I; 11.02% of Fe; 2.64% of Mn; 198.4 mg/kg of Se; 11.02% of Zn.

⁴Corn AF was corn naturally contaminated with 20 µg AF/kg and 1.6 mg fumonisin/kg.

⁵Aflatoxin in diets were analyzed by a commercial laboratory (Food and Drug Protection Division Laboratory, NC).