Table 6.
Closest relatives and relative abundance of bands from 16S rRNA gene V3 PCR-DGGE fingerprint of adhesive microbiota for the control and 0.6% groups.
| Phylum | Band No. | Closest relative (BLAST search) | Identity, % | Relative abundance1, % |
|
|---|---|---|---|---|---|
| Control | 0.60% nucleotides | ||||
| Firmicutes | 1 | Lachnospiraceae bacterium (GU723317.1) | 99 | 6.4 ± 1.0 | 6.0.4 ± 0.5 |
| 3 | Streptococcus pleomorphus (NR_044660.1) | 100 | 18.1 ± 0.7 | 21.1 ± 2.0 | |
| 4⁎ | Clostridium acidurici (HE582772.1) | 98 | 9.9 ± 0.3 | 7.9 ± 0.6 | |
| 8 | Clostridium beijerinckii (AB600543.1) | 100 | 3.5 ± 0.8 | 1.4 ± 0.7 | |
| 9 | Robinsoniella peoriensis (JN642224.1) | 98 | 3.3 ± 0.5 | 2.6 ± 0.3 | |
| 10 | Faecalibacterium prausnitzii (HQ457030.1) | 100 | 4.1 ± 0.4 | 4.4 ± 0.6 | |
| 11 | Anaerostipes hadrus (NR_104799.1) | 100 | 3.3 ± 0.3 | 1.0 ± 1.0 | |
| 12⁎⁎⁎ | Lachnospiraceae bacterium (EU728751.1) | 100 | 5.3 ± 0.5 | 0 | |
| 14 | Streptococcus sp. (FJ611790.1) | 100 | 24.0 ± 4.0 | 28.1 ± 0.7 | |
| 10 + 112 | – | – | 7.4 ± 0.2 | 5.4 ± 0.9 | |
| 1 + 10+11 + 12⁎⁎⁎2 | – | – | 19.1 ± 0.5 | 11.8 ± 0.4 | |
| Proteobacteria | 5 | Escherichia coli (KC138767.1) | 100 | 1.4 ± 1.4 | 3.9 ± 0.2 |
| 6⁎⁎⁎ | Plesiomonas sp. (FJ405284.1) | 100 | 0 | 3.6 ± 1.0 | |
| 7⁎ | Plesiomonas shigelloides (JQ582978.1) | 99 | 6.3 ± 0.6 | 3.9 ± 0.5 | |
| 13 | Plesiomonas sp. (AB192384.1) | 100 | 7.4 ± 1.0 | 8.0 ± 0.6 | |
| Bacteroidetes | 2 | Bacteroides propionicifaciens (AB264624.2) | 100 | 7.2 ± 0.2 | 7.7 ± 0.5 |
⁎ and ⁎⁎⁎ represent significant differences P < 0.05 and P < 0.001, respectively, in relative abundance of the band(s) between the control and 0.60% groups.
1
Relative abundance data are means of triplicate and presented as means ± SEM, n = 3.
2
Cumulative relative abundance of putative butyrate-producing species in the adhesive intestinal microbiota.