. 2015 Aug 19;1(3):213–219. doi: 10.1016/j.aninu.2015.08.003

Table 5.

Hematological parameters, serum activity of enzymes and metabolite concentrations in serum of broilers supplemented with an antibiotic growth promoter or a phytogenic feed additive (PFA) and infected with Salmonella and Escherichia coli.

Parameter	Dietary treatment¹			SEM	P-value
Parameter	NC	PC	PFA	SEM	P-value
Hemoglobin, g/L	102.8	106.6	106.3	0.4	0.717
Total erythrocytes , 10¹²/L	3.54	3.66	3.64	0.01	0.724
Total leukocytes , 10⁹/L	46.6^a	69.4^b	59.8^b	2.66	0.0001
Differential leukocyte counts, 10⁹/L
Heterophils	11.9^b	11.2^b	5.4^a	0.74	0.0001
Lymphocytes	29.3^a	48.6^b	47.7^b	2.41	0.0001
Monocytes	0.87	1.23	0.81	0.09	0.098
Eosinophils	4.41^a	7.24^b	4.09^a	0.54	0.025
Basophils	0.14^a	1.09^ab	1.71^b	0.22	0.007
Heterophil:lymphocyte ratio	0.413^c	0.234^b	0.113^a	0.03	0.0001
Serum enzyme activity, µ/L
Alkaline phosphates	2.5^a	3.79^a	6.23^b	0.49	0.003
Acid phosphatase	4.86	3.91	5.24	0.34	0.268
Amylase	643.3	677.1	727.5	32.65	0.591
Serum metabolites
Total protein, g/L	33.19	34.19	35.59	0.58	0.243
Albumin, g/L	18.98	18.99	20.44	0.44	0.303
Glucose, mmol/L	9.86	10.03	10.28	0.08	0.131

^a,b,c Within a row, means with dissimilar letters varied significantly (P < 0.05).

NC is negative control, the basal diet; PC is the basal diet supplemented with bacitracin methylene disalicylate, 500 mg/kg; PFA is the basal diet supplemented with a phytogenic feed additive, 150 mg/kg. Means of 8 replicate cages (n = 4 birds in each cage). Mortality was not there in any of the treatments and hence is not mentioned (means of 8 birds from each dietary group).