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. 2018 May 4;5:134. doi: 10.3389/fmed.2018.00134

Figure 4.

Figure 4

Myeloperoxidase (MPO) activity is important for the membrane translocation and activation of αMβ2 integrin. (A,B) Wild-type (WT) mouse neutrophils were preincubated with vehicle or 500 µM 4-ABAH, an MPO inhibitor, and stimulated with formyl-methionyl-leucyl-phenylalanine (fMLP) or PMA. The surface level of αMβ2 integrin was measured by flow cytometry. (C–F) Human neutrophils were preincubated with vehicle or 500 µM 4-ABAH and stimulated with fMLP or PMA, followed by flow cytometric analysis using conformation-specific antibodies against total [ICRF44 (C,D)] or activated human αMβ2 [CBRM1/5 (E,F)]. Data are shown as mean ± SD. *P < 0.05 or **P < 0.01 versus WT after Student’s t-test.