Table 1.
Primers used for mutants construction.
| Primer names | Primer sequences (5′ to 3′) |
|---|---|
| pJA2-nc117-F | TGCTCTAGAAACTTAAGAGCGAAGTAAGT |
| pJA2-nc117-R | CGCGGATCCGAAAATGGAAAGAAGACGCT |
| Δnc117-F1 | ACCCCCGATGATTTTGCCAT |
| Δnc117-R1 | CCAGTGGCTTCTGTTTCTATCAGCTTTCGCTCTTAAGTTCATGCC |
| Δnc117-F2 | AGCTGATAGAAACAGAAGCCACTGG |
| Δnc117-R2 | TTACGCCCCGCCCTGCCACTCATCG |
| Δnc117-F3 | CGATGAGTGGCAGGGCGGGGCGTAAATACTTTCCGAGCCCAGAAT |
| Δnc117-R3 | AATAGCTGTCGGCGATGGAG |
| pJA2-slr0007-F | TGCTCTAGAATGGCTCTTTCCCCCGCAGA |
| pJA2-slr0007-R | CGCGGATCCCTATTTGTCTAGGTCTTGAA |
| Δslr0007-F1 | CCCTGCCATTCAATCCGTCT |
| Δslr0007-R1 | CCAGTGGCTTCTGTTTCTATCAGCTAGAGTTGAAAAAGTAGAAAC |
| Δslr0007-F2 | AGCTGATAGAAACAGAAGCCACTGG |
| Δslr0007-R2 | TTACGCCCCGCCCTGCCACTCATCG |
| Δslr0007-F3 | CGATGAGTGGCAGGGCGGGGCGTAACTTAAAATGAGAAGCTAACT |
| Δslr0007-R3 | CAAGTTGATGCAGAGCGTGG |
| pJA2-slr2126-F | TGCTCTAGAATGTTTTTCATGCAAAATACTAAGT |
| pJA2-slr2126-R | CGCGGATCCTCACAAAGTTAGTTTTTGATCGAGA |
| Δslr2126-F1 | TTGTAATGGCCCCAGCTTGT |
| Δslr2126-R1 | AGCTGATAGAAACAGAAGCCACTGGGAATTAATAATATTCTTTGT |
| Δslr2126-F2 | AGCTGATAGAAACAGAAGCCACTGG |
| Δslr2126-R2 | TTACGCCCCGCCCTGCCACTCATCG |
| Δslr2126-F3 | CGATGAGTGGCAGGGCGGGGCGTAATACCCCCTACTACAAATCCC |
| Δslr2126-R3 | TCCGCTTCTTGGGACTGTTC |
| pJA2-sll1830-F | TGCTCTAGAATGATCAATCGTCAGGACCT |
| pJA2-sll1830-R | CGCGGATCCTCAGTAGCGTAAAACCAAGG |
| Δsll1830-F1 | CTTTCCATGGCCGCTAAACG |
| Δsll1830-R1 | CCAGTGGCTTCTGTTTCTATCAGCTGGCCATTGATTAAGTTAAGG |
| Δsll1830-F2 | AGCTGATAGAAACAGAAGCCACTGG |
| Δsll1830-R2 | TTACGCCCCGCCCTGCCACTCATCG |
| Δsll1830-F3 | CGATGAGTGGCAGGGCGGGGCGTAATTTGTCTTTACATAGGTCGA |
| Δsll1830-R3 | CCCGAATTTCTGTGCTCCCA |
| pJA2-slr2108-F | TGCTCTAGAATGGGAGTGGATGGGATGAC |
| pJA2-slr2108-R | CGCGGATCCTTAGGTCGTACATAAGTGCC |
| Δslr1501-F1 | GCATTGGGCAGTTGTAAGCC |
| Δslr1501-R1 | CCAGTGGCTTCTGTTTCTATCAGCTGACCCCTTGGGGAAAGTTTT |
| Δslr1501-F2 | AGCTGATAGAAACAGAAGCCACTGG |
| Δslr1501-R2 | TTACGCCCCGCCCTGCCACTCATCG |
| Δslr1501-F3 | CGATGAGTGGCAGGGCGGGGCGTAAAAAAGATAACTACCATGTTT |
| Δslr1501-R3 | ACCTAGTTCCATCACCCCGA |
| Δsll0784-F1 | TCGGCTTTTCCGGGCATAAT |
| Δsll0784-R1 | CCAGTGGCTTCTGTTTCTATCAGCTGTACAAAAATTTAAGGATTA |
| Δsll0784-F2 | AGCTGATAGAAACAGAAGCCACTGG |
| Δsll0784-R2 | TTACGCCCCGCCCTGCCACTCATCG |
| Δsll0784-F3 | CGATGAGTGGCAGGGCGGGGCGTAAAACGACTGGAATAGTTTCAG |
| Δsll0784-R3 | AAGGAAACTTCGCTTCTACATTGA |
| pJA2-up500-F | GTTCCGCGCACATTTCCCCGA |