Figure 2.

NF‐κB mediates increased SCN9A expression and oncogene‐induced senescence. (a,b) HEC‐TM cells were infected with control or mIKBA‐expressing retroviral vectors and puromycin selected. The day after seeding, they were treated daily with 4‐OHT for 4 d and RNAs were prepared, and (a) mIKBA expression and (b) SCN9A expression were measured by RT‐qPCR. Their relative mRNA expressions were normalized against GAPDH mRNA levels. (c) Immunofluorescence staining against SCN9A was performed. (d) Cells were fixed and stained using crystal violet. (e) Cells were fixed and stained for SA‐β‐Gal activity. (f,g) RNAs were prepared, and the mRNA levels of (f) IL8 and (g) IL6 were analyzed by RT‐qPCR. Their expressions were normalized against GAPDH mRNA. The experiments shown are representative of at least two biological repeats. Statistical analysis was performed with the Student's t test, ** means p < .01, ***p < .001