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. 2018 Mar 23;17(3):e12753. doi: 10.1111/acel.12753

Figure 4.

Figure 4

AGO2 and DROSHA mRNAs are methylated in PBMCs. (a, b) Number of methylated fragments (FPKM) on DROSHA and AGO2 mRNAs in young and old PBMCs. Data in A are statistically significant (p = .047, Student's t‐test) (c) Relative levels of AGO1‐4, DROSHA, DGCR8, and DICER1 mRNAs in young and old PBMCs. (d) Relative abundance of METTL14,METTL3,WTAP,ALKBH5,FTO, and YTHDF2 mRNAs in young and old PBMCs. All p‐values were calculated using Student's t‐test. (e) AGO2 protein level analyzed by Western blot in young and old PBMCs. (f) Number of AUF1 and HuR binding sites on the indicated mRNAs from PAR‐CLIP analysis (Yoon et al., 2014). (g) Relative expression level (FPKM) of the indicated mRNAs from total RNA‐seq after depletion of either AUF1 or HuR