Figure 1.

Expression of senescence‐associated genes in control or calorie restricted (CR) mouse colon. RNA was extracted from the proximal (a), medial (b), or distal (c) colon of mice with 24 or 12 hr ad libitum access to food (24AL and 12AL, respectively) or mice under 10%, 20%, 30%, or 40% calorie restriction (10CR, 20CR, 30CR, and 40CR, respectively). mRNA encoding p16, p21, Il1a, Mmp9, and Cxcl1 were quantified by qRT–PCR. mRNA encoding tubulin was used as internal control. N = 12–18. *p < .05