Figure 2.

Expression of senescence‐associated genes in control or calorie restricted (CR) human colon. RNA was extracted from the sigmoid portion of the colon of human volunteers. The groups were as follows: CR, volunteers of average age 61.7 ± 8.4 under <15% calorie restriction; WD‐o, volunteers of average age 62.4 ± 8.5 on normal Western diet; WD‐y, volunteers of average age 24.3 ± 2.0 on normal Western diet. A summary is prided in a. mRNA encoding p16 (b), p21 (c), Il6 (d) and other SASP factors Cxcl1, IL‐8, Il1a, and Mmp9 (e) were quantified by qRT–PCR. mRNA encoding tubulin was used as internal control. In E, dotted line represents the baseline value of WD‐y samples. N = 6, WD‐y; N = 12, CR and WD‐o. *p < .05; **<.01