Skip to main content
. 2018 Apr 23;7(4):32. doi: 10.3390/cells7040032

Figure 2.

Figure 2

CDK5RAP2 is present at mitotic spindle poles. (A) Immunofluorescence microscopy of AX2 control cells in interphase and mitosis (as indicated) stained with rabbit anti-CDK5RAP2 and rat anti-α-tubulin. DNA staining with DAPI is shown in the merged image. Secondary antibodies were anti-rabbit AlexaFluor 488 and anti-rat AlexaFluor 568. Cells were fixed with glutaraldehyde. Maximum intensity projections of deconvolved images. To allow comparison of labeling intensities in mitotic vs. interphase cells, the maximum display intensity of the green channel was set using neighboring interphase cells. Bar = 2 µm. (B) Immunoblot of a nuclear extract of untransformed AX2 cells stained with anti-CDK5RAP2/anti-rabbit-alkaline phosphatase. Color detection was performed with nitroblue tetrazolium chloride (NBT) and bromo-chloro-indolyl-phosphate (BCIP).