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. 2018 Apr 23;7(4):32. doi: 10.3390/cells7040032

Figure 7.

Figure 7

Overexpressed GFP-CDK5RAP2 is concentrated in discrete foci in the cytosol or nucleus. Immunofluorescence microscopy of GFP-CDK5RAP2 cells stained with the indicated antibodies. DNA was stained with DAPI. Secondary anti-rabbit/rat/mouse antibodies were conjugated with AlexaFluor 568. Cells were fixed with methanol. Maximum intensity projections of deconvolved images are displayed, except for the GFP-CDK5RAP2 sample co-stained for NE81 (lamin, nuclear envelope marker, [34]), where only the optical section through the center of the nucleus is shown, to show that there are indeed GFP-CDK5RAP2 foci inside the nucleus. Bar = 2 µm.