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. 2018 Apr 23;7(4):32. doi: 10.3390/cells7040032

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© 2018 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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CDK5RAP2RNAi and CP148RNAi cause similar centrosome disruptions. Immunofluorescence microscopy of CDK5RAP2-GFP/CP148RNAi (A) and GFP-TubA/CDK5RAP2RNAi (B) cells stained with the indicated antibodies. DNA-staining with DAPI is shown in the merged images. In (A) primary and secondary antibodies were anti-α-tubulin/anti-rat-Atto647n and anti-Cep192 (core marker; arrowhead)/anti-rabbit-AlexaFluor 568, in (B) anti-CP55 (core marker; arrowhead)/anti-rat-AlexaFluor 568 and anti-CP148/anti-rabbit-Alexa 647. Cells were fixed with methanol. Maximum intensity projections of deconvolved images are displayed. Bars = 2 µm.