Figure 2.

Determination of the cellular localization of EGFP plasmids in transiently transfected HeLa cells. Immunocytochemistry was performed on HeLa cells transfected with EGFP fusion proteins. Cells were stained with an anti-lamin B1 antibody. Chromatin was stained with DAPI. Representative images of lamin B1 staining in the indicated cells. (A) NLS directs the protein to the cell nucleus; (B) The farnesylated CaaX motif, along with the NLS, targets the EGFP–NLS-59-R-CSIM and EGFP–NLS-PG-9-CSIM proteins to the nuclear envelope (NE) and EGFP–NLS-20-CSIM protein to the NE and cytoplasm and membrane; (C) Proteins carrying the C661S mutation within the CaaX motif exhibit an intra-nucleoplasm diffusion pattern. Scale bar, 10 µm.