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. 2018 Apr 4;8(4):52. doi: 10.3390/ani8040052

Figure 2.

Figure 2

Setup of the gas chamber for Experiment 2. Setup was similar to Experiment 1 except there were two cameras (1) and two acrylic glass observation windows (2). Instead of a ventilator, the vacuum pump created negative pressure and the air it took out was routed through a tube (3) to the outside. The on/off valves (4), tubing, and tanks (5) were similar. However, there was only one tube (6) that went to one mass flow controller and then into the box, instead of two mass flow controllers as was used in experiment 1.