Figure 3.

Loss of PD‐1 resulted in decreased expression of CD103 as well as reduced CD69⁺CD103⁺ co‐expression on CD8⁺ T‐cells. CD8⁺ T‐cells from uninfected PD‐1 KO mice were either left unstimulated or stimulated with anti‐CD3 Ab and co‐cultured with mixed glial cells. Cells were collected at 48 h of culture and analyzed for the expression of CD103 and co‐expression of CD69⁺CD103⁺ on gated CD8⁺ T‐cells. (A) Representative contour plots show analysis of KLRG1 and CD103 expression on gated CD8⁺ T‐cells obtained from PD‐1 KO mice. (B) Data show frequency of CD103 expressing CD8⁺ T‐cells obtained from both WT (presented in Figure 2) and PD‐1 KO animals. (C) Flow cytometry plot shows co‐expression of CD69 and CD103 on gated CD8⁺ T‐cells from PD‐1 KO mice. (D) Pooled data present the frequency (mean ± SD) of co‐expressed CD69 and CD103 cells among different groups of mice under the indicated culture conditions. Data are representative of three separate experiments. *p < 0.05 **p < 0.01 ***p < 0.001.