Table 5.
Antibodies and instrument configuration used for antigen detection for Figure 2B and C
| Antigen | Source | Clone | ng/test | Fluorochrome | Excitation (nm) | Longpass Dichroic Mirror | Bandpass filter |
|---|---|---|---|---|---|---|---|
| CD45RO | Biolegend | UCHL1 | 2500 | Brilliant violet 421 | 405 | blank | 450/50 |
| CD4 | Biolegend | OKT4 | 150 | Brilliant violet 510 | 405 | 505LP | 525/50 |
| CD45RA | Biolegend | HI100 | 400 | AF488 | 488 | 505LP | 530/30 |
| CD3 | eBioscience | UCHT1 | 500 | PerCP‐Cy5.5 | 488 | 685LP | 695/40 |
| CD8 | Biolegend | HIT8a | 2500 | AF700 | 640 | 685LP | 710/50 |
| Viability marker | eBioscience | Cat# 65‐0865 | 1:1000 | eF780 | 640 | 755LP | 780/60 |
| FoxP3 | BD | 259D/C7 | 500 | PE | 561 | blank | 582/15 |
| CD3 | BD | UCHT1 | 500 | BUV395 | 355 | blank |