Table 1. Hybridization Parameters^a.

sample	kd [s–1]	ka × 10–6 [M–1 s–1]	Kd [nM]	ΔG [kJ/mol]
9 nt	0.180 ± 0.012	2.5 ± 0.5	72 ± 16	40.5 ± 0.6
10 nt	0.009 ± 0.002	2.1 ± 0.4	4.2 ± 1.8	47.5 ± 1.1

^aFor 9 and 10 nt determined by SI-FCS and titration of free ssDNA. Errors are given by the 95% confidence bounds of the fit.