Table 5.
Short-chain fatty acid (SCFA) concentration and profile in the cecal digesta of rats fed experimental diets *.
| SCFA Concentration, μmol/g Digesta | SCFA Profile, Percent of Total SCFA | ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| C2 | C3 | C4i | C4 | C5i | C5 | PSCFA | SCFA | C2 | C3 | C4 | |
| Group (n = 8) | |||||||||||
| C | 94.1 | 22.2 | 2.11 | 15.4 | 2.47 | 2.07 | 6.65 | 138 | 67.7 | 16.2 | 11.2 |
| F | 73.1 | 13.8 | 1.82 | 7.36 | 2.50 | 2.31 | 6.63 | 101 | 72.2 | 13.8 | 7.32 |
| C + ME | 101 | 23.0 | 2.14 | 16.3 | 2.16 | 2.51 | 6.81 | 147 | 68.6 | 15.7 | 11.0 |
| F + ME | 104 | 20.8 | 2.47 | 13.0 | 2.59 | 2.63 | 7.70 | 146 | 71.3 | 14.4 | 8.98 |
| C + DE | 104 | 21.7 | 3.03 | 14.7 | 3.11 | 2.74 | 8.88 | 149 | 69.7 | 14.5 | 9.81 |
| F + DE | 90.4 | 19.6 | 4.07 | 10.2 | 1.92 | 2.35 | 8.33 | 128 | 70.8 | 15.1 | 7.97 |
| SEM | 2.639 | 0.757 | 0.327 | 0.617 | 0.162 | 0.091 | 0.413 | 3.722 | 0.575 | 0.360 | 0.310 |
| Extract (E) | |||||||||||
| - (without) | 83.6 b | 18.0 b | 1.96 | 11.4 b | 2.49 | 2.19 | 6.64 | 120 b | 70.0 | 15.0 | 9.26 |
| ME | 103 a | 21.9 a | 2.30 | 14.6 a | 2.38 | 2.57 | 7.25 | 146 a | 69.9 | 15.0 | 10.0 |
| DE | 97.2 a | 20.6 a,b | 3.55 | 12.4 b | 2.51 | 2.54 | 8.61 | 135 a | 70.3 | 14.8 | 8.89 |
| p value | 0.004 | NS | NS | 0.013 | NS | NS | NS | 0.002 | NS | NS | NS |
| Diet (D) | |||||||||||
| Corn starch | 99.7 a | 22.3 a | 2.43 | 15.5 a | 2.58 | 2.44 | 7.45 | 145 a | 68.7 b | 15.5 | 10.7 a |
| Fructose | 89.2 b | 18.1 b | 2.78 | 10.2 b | 2.34 | 2.43 | 7.55 | 125 b | 71.4 a | 14.4 | 8.09 b |
| p value | 0.024 | 0.002 | NS | <0.001 | NS | NS | NS | 0.002 | 0.020 | NS | <0.001 |
| Interaction E × D | |||||||||||
| p value | NS | NS | NS | NS | NS | NS | NS | NS | NS | NS | NS |
* C, control fed a diet with 65.8% corn starch; F, fed a diet with 65.0% fructose (F) added at the expense of corn starch; C + ME, fed a corn starch diet with a monomeric ET-rich extract; F + ME, fed a fructose diet with a monomeric ET-rich extract; C + DE, fed a corn starch diet with a dimeric ET-rich extract; F + DE, fed a fructose diet with a dimeric ET-rich extract. a,b Mean values within a column with different superscript letters were shown to be significantly different (p < 0.05); differences among the groups (C, F, C + ME, F + ME, C + DE, and F + DE) are indicated with superscripts only in the case of a statistically significant interaction E × D (p < 0.05). PSCFA, putrefactive SCFA (the sum of isobutyric, isovaleric and valeric acids); acids: C2, acetic; C3, propionic; C4i, isobutyric; C4, butyric; C5i, isovaleric; C5, valeric.