Table 11.
Multiple regression analyses to find the important protein structural variables for predicting chemical profiles of canola seeds.
| Predicted Variables (Y) | Variable (s) Selection (Variables Left in the Model with p < 0.05) | Prediction Equation Test Model: Y = a + b1 × x1 + b2 × x2… | R2 Value | * RSD | p Value |
|---|---|---|---|---|---|
| ** DM% | *** Height left in the model | DM% = 97.44 − 2.01 × Height | 0.30 | 0.80 | 0.002 |
| Ash (%DM) | No variable met the 0.05 significance level for entry into the model | ||||
| EE (%DM) | No variable met the 0.05 significance level for entry into the model | ||||
| FA (%DM) | No variable met the 0.05 significance level for entry into the model | ||||
| Protein profile | |||||
| CP (%DM) | No variable met the 0.05 significance level for entry into the model | ||||
| SCP (%DM) | Peak area and AI left in the model | SCP (%DM) = 7.43 + 0.56 × Peak area − 0.97 × AI | 1.55 | 0.62 | 0.002 |
| SCP (%CP) | Peak area and AI left in the model | SCP (%CP) = 33.81 + 2.69 × Peak area − 4.74 × AI | 0.38 | 5.76 | 0.001 |
| NPN (%DM) | No variable met the 0.05 significance level for entry into the model | ||||
| NPN (%CP) | No variable met the 0.05 significance level for entry into the model | ||||
| NPN (%SCP) | Peak area and AI left in the model | NPN (%SCP) = 113.86 − 10.43 × Peak area + 19.24 × AI | 0.39 | 20.86 | 0.001 |
| NDICP (%DM) | Peak area and AI left in the model | NDICP (%DM) = 2.10 − 0.13 × Peak area +0.24 × AI | 0.51 | 0.20 | <0.001 |
| NDICP (%CP) | Peak area and AI left in the model | NDICP (%CP) = 13.50 − 0.55 × Peak area +1.00 × AI | 0.51 | 0.87 | <0.001 |
| ADICP (%DM) | No variable met the 0.05 significance level for entry into the model | ||||
| ADICP (%CP) | No variable met the 0.05 significance level for entry into the model | ||||
| CHO profile | |||||
| CHO (%DM) | No variable met the 0.05 significance level for entry into the model | ||||
| Sugar (%DM) | No variable met the 0.05 significance level for entry into the model | ||||
| Sugar (%NFC) | No variable met the 0.05 significance level for entry into the model | ||||
| NDF (%DM) | No variable met the 0.05 significance level for entry into the model | ||||
| ADF (%DM) | No variable met the 0.05 significance level for entry into the model | ||||
| ADF (%NDF) | No variable met the 0.05 significance level for entry into the model | ||||
| ADL (%DM) | Height left in the model | ADL (%DM) = 3.76 + 0.82 × Height | 0.14 | 0.54 | 0.042 |
| ADL (%NDF) | Height left in the model | ADL (%NDF) = 20.43 + 5.61 × Height | 0.21 | 2.82 | 0.010 |
| Cell (%DM) | No variable met the 0.05 significance level for entry into the model | ||||
| NFC (%DM) | No variable met the 0.05 significance level for entry into the model | ||||
| NFC (%CHO) | No variable met the 0.05 significance level for entry into the model | ||||
| NSC (%DM) | No variable met the 0.05 significance level for entry into the model |
Notes: * RSD, residual standard deviation. ** DM, dry matter; EE, ether extract; FA, fatty acid; CP, crude protein; SCP, soluble crude protein; NPN, non-protein nitrogen; NDICP, neutral detergent-insoluble crude protein; ADICP, acid detergent-insoluble crude protein; CHO, total carbohydrate; NDF, neutral detergent fiber; ADF, acid detergent fiber; ADL, acid detergent lignin; Cell, cellulose; NFC, non-fiber carbohydrate; NSC, non-structural carbohydrate. *** Protein structural spectral parameters; Height, Height ratios of amide I and II; Peak area, Amide I and II peak area; AI, Amide I area.