Table 5.
Antiradical activity of protein hydrolysates after gastric-intestinal digestion (mean ± standard deviation).
| Sample | Aging Time (Days) | WSF | SSF | ||||
|---|---|---|---|---|---|---|---|
| -NH2 (µM/mL) | Radical Scavenging (%) | IC50 (µM/mL) | -NH2 (µM/mL) | Radical Scavenging (%) | IC50 (µM/mL) | ||
| C | 28 | 21.08 a,A,B ± 1.24 | 86.43 a,A ± 1.00 | 0.16 a,A ± 0.01 | 10.61 a,A ± 2.39 | 82.31 a,A ± 0.64 | 0.17 a,A ± 0.01 |
| 90 | 18.63 a,A ± 0.42 | 93.49 b,A ± 1.73 | 0.35 b,A ± 0.02 | 15.36 b,A,B ± 1.88 | 85.71 b,A ± 0.50 | 0.28 b,A ± 0.01 | |
| 180 | 28.88 b,A ± 1.32 | 96.01 c,A ± 0.68 | 0.17 a,A,B ± 0.02 | 12.88 c,A ± 0.25 | 87.06 c,A ± 0.90 | 0.13 c,A ± 0.02 | |
| 270 | 18.68 a,A ± 0.86 | 94.93 c,A ± 0.62 | 0.22 a,A ± 0.01 | 7.30 d,A ± 0.84 | 95.86 d,A ± 0.37 | 0.14 a,c,A ± 0.01 | |
| 360 | 31.39 b,A,B ± 2.41 | 91.73 d,A ± 0.76 | 0.51 c,A ± 0.06 | 15.87 b,A ± 2.28 | 94.84 d,A ± 1.96 | 0.14 a,c,A,B ± 0.01 | |
| LOCK | 28 | 20.88 a,A,B ± 1.30 | 88.63 a,B ± 0.72 | 0.19 a,B ± 0.02 | 10.46 a,A ± 1.49 | 72.81 a,B ± 1.91 | 0.15 a,B ± 0.01 |
| 90 | 21.24 a,B ± 1.27 | 96.67 b,B ± 0.69 | 0.32 b,A ± 0.01 | 16.56 b,A ± 1.45 | 89.34 b,B ± 0.74 | 0.23 b,B ± 0.01 | |
| 180 | 25.68 b,B ± 2.01 | 96.33 b,d,A ± 0.55 | 0.19 a,A ± 0.01 | 18.81 b,B ± 2.26 | 90.05 b,B ± 1.32 | 0.11 c,A ± 0.01 | |
| 270 | 21.31 a,B ± 0.05 | 97.83 c,B ± 0.50 | 0.21 a,A ± 0.01 | 7.34 c,A ± 0.01 | 91.01 b,c,B ± 0.68 | 0.14 a,c,A ± 0.01 | |
| 360 | 27.94 b,A ± 1.10 | 95.58 d,B ± 0.66 | 0.43 c,B ± 0.01 | 16.40 b,A ± 0.50 | 91.82 c,B ± 1.19 | 0.12 c,A ± 0.01 | |
| BAUER | 28 | 19.00 a,B ± 1.17 | 85.94 a,A ± 0.98 | 0.14 a,A,C ± 0.01 | 10.63 a,A ± 1.17 | 72.45 a,B ± 1.03 | 0.14 a,C ± 0.01 |
| 90 | 18.59 a,A ± 0.14 | 94.41 b,A ± 0.38 | 0.41 b,B ± 0.02 | 12.40 a,B ± 2.07 | 90.95 b,c,C ± 0.62 | 0.20 b,C ± 0.01 | |
| 180 | 37.16 b,C ± 2.04 | 98.06 c,B ± 0.23 | 0.13 a,B ± 0.01 | 18.12 b,B ± 2.16 | 90.14 b,B ± 1.56 | 0.12 a,c,A ± 0.05 | |
| 270 | 22.76 c,C ± 1.21 | 95.59 d,A ± 1.09 | 0.35 c,C ± 0.01 | 10.78 a,B ± 0.36 | 92.15 cC ± 0.54 | 0.13 a,c,A ± 0.01 | |
| 360 | 33.28 d,B ± 1.15 | 95.71 d,B ± 1.20 | 0.47 d,A,B ± 0.01 | 19.94 b,B ± 2.35 | 88.60 d,C ± 1.54 | 0.12 c,A,C ± 0.01 | |
| BB12 | 28 | 21.61 a,A ± 1.35 | 89.52 a,B ± 1.44 | 0.13 a,C ± 0.02 | 10.78 a,A ± 0.45 | 78.93 a,C ± 1.28 | 0.19 a,D ± 0.01 |
| 90 | 19.35 a,A ± 0.09 | 98.56 bC ± 0.23 | 0.32 b,A ± 0.01 | 14.87 b,A,B ± 2.18 | 92.62 b,c,D ± 0.85 | 0.22 b,B,C ± 0.01 | |
| 180 | 31.80 b,A ± 0.74 | 96.95 c,C ± 0.56 | 0.13 a,B ± 0.01 | 17.71 c,B ± 0.79 | 91.86 b,C ± 0.50 | 0.11 c,A ± 0.02 | |
| 270 | 26.26 c,D ± 0.02 | 98.08 b,c,B ± 0.33 | 0.51 c,B ± 0.02 | 7.04 d,A ± 0.61 | 93.33 c,D ± 0.52 | 0.11 c,B ± 0.01 | |
| 360 | 28.40 c,A ± 2.89 | 94.69 d,B ± 1.29 | 0.42 d,B ± 0.01 | 18.69 c,C ± 0.45 | 93.55 c,A,B ± 1.54 | 0.15 d,B ± 0.01 | |
-NH2, the content of primary amino groups; WSF, water-soluble fraction; SSF, salt-soluble fraction; C, control sample; LOCK, sample inoculated with Lactobacillus rhamnosus LOCK900; BAUER, sample inoculated with Lactobacillus acidophilus Bauer Ł0938; BB12, sample inoculated with Bifidobacterium animalis ssp. lactis BB-12; a–d Within the same treatment, means followed by a common letter do not differ significantly (p < 0.05); A–D Within the same aging time, means followed by a common letter do not differ significantly (p < 0.05).