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. Author manuscript; available in PMC: 2019 May 1.
Published in final edited form as: J Am Soc Mass Spectrom. 2018 Apr 17;29(5):807–816. doi: 10.1007/s13361-018-1914-1

Figure 4. Neuropeptidomics analyses of proenkephalin-derived peptides without and with trypsin or V8 protease digestion.

Figure 4

To identify peptides by neuropeptidomics, the soluble low molecular weight soluble fraction of human chromaffin granules was undigested, or digested with trypsin or V8 protease for nano-LC-MS/MS identification of peptides. Peptides derived from proenkephalin (PE) were mapped to PE, illustrated by colored lines: QTOF-no enzyme (purple), QTOF-trypsin (bright violet), QTOF-V8 (lavender), Trap-no enzyme (olive), Trap-trypsin (turquoise blue), Trap-V8 (bright green). Active enkephalin neuropeptides within PE are shown in yellow, and dibasic cleavage sites are highlighted by boxes. Phosphorylated peptides are indicated (light blue with phosphorylation site indicated by dark blue square). Hyphens at the end of some lines indicate peptides that were split between two lines in the figure.