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. 2018 May;58(5):658–667. doi: 10.1165/rcmb.2016-0344OC

Figure 3.

Figure 3.

Eact mediates its apoptotic effects via Ano1. (A) RLMVECs were transfected with either scrambled or Ano1-targeted siRNA, and Ano1 expression was assessed using immunoblot (n = 3, *P < 0.05). (B) RLMVECs were transfected with scrambled or Ano1 targeted siRNA and then treated with vehicle or 10 μM Eact for 24 hours. Expression of cleaved caspase-3 and vinculin (loading control) was then evaluated by immunoblot (n = 3, ANOVA with Tukey multiple comparison, *P < 0.05). (C) RLMVECs were transfected with scrambled or Ano1-targeted siRNA, and cells were counted after treatment with vehicle or 10 μM Eact for 24 hours (n = 3, ANOVA with Tukey multiple comparison, *P < 0.05). Data are presented as mean (±SEM).