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. 2018 May;58(5):585–593. doi: 10.1165/rcmb.2017-0186OC

Figure 3.

Figure 3.

PHGDH and SHMT2 are induced in fibrotic mouse lungs after bleomycin instillation. (A) The protein expression of PHGDH and SHMT2 was determined by Western blot in whole-lung homogenates 14 days after intratracheal instillation of bleomycin or PBS (control). (B) Representative histological assessment of PHGDH and SHMT2 protein expression in the lungs of mice 14 days after intratracheal instillation of bleomycin. Scale bars: 100 μm. Low and high power ×200 and ×400, respectively. (C) Schematic of experimental timeline. ROSAmT/mG; Col1a2-CreERT2+/− were injected with tamoxifen five times before intratracheal instillation of bleomycin. At 14 days after bleomycin, mice were killed and lungs were either imaged or digested to sort enhanced GFP (eGFP)-positive cells. (D) Representative image of tdTomato and eGFP expression in lungs of ROSAmT/mG; Col1a2-CreERT2+/− mice 14 days after instillation of either bleomycin or PBS (control). Scale bar: 25 μm. Magnification ×400. (E) GFP-positive lung cells were collected from ROSAmT/mG; Col1a2-CreERT2+/− mice 14 days after instillation of bleomycin or PBS (control), and the expression of Phgdh, Shmt2, and Col1a1 mRNAs were measured by qRT-PCR (mean ± SEM, n = 4). *P < 0.05, **P < 0.01.