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. 2018 May;58(5):566–574. doi: 10.1165/rcmb.2017-0324MA

Figure 3.

Figure 3.

Macrophage chemotactic protein-1 (MCP-1)/Ccl2 expression by MLE-15/HPS cell lines. (A) Ccl2 qRT-PCR using RNA from WT MLE-15 cells and MLE-15/HPS1, MLE-15/HPS2, and MLE-15/HPS3 cells cultured for 24 hours (n = 7 experiments/triplicate samples; normalized to Rn18s and Gapdh and reported as relative quantity compared to WT/RQ; mean ± SD). (B and C) MCP-1 expression in cell culture media evaluated by ELISA and Ccl2 qPCR using (B) primary mouse AT2 cells from WT/C57BL/6 (mAT2 WT) and pallid (mAT2 pallid) mice (n = 2 experiments/triplicate samples; qPCR normalized to Rn18s and Gapdh and reported as RQ; mean ± SD) and (C) WT MLE-15 and MLE-15/HPS9 cells. (n = 3 experiments/triplicate samples; qPCR normalized to Rn18s and Gapdh and reported as RQ; mean ± SD).