Fig. 8.

Contextual and cued fear conditioning test. a-f Effects of Rapgef2 deficiency. Conditioned stimulus (CS; 55 dB white noise for 30 s) and aversive unconditioned stimulus (UCS; 0.3 mA foot shock for 2 s) were given during the indicated periods. Percentages of freezing time during the conditioning phase (a), context testing and cued testing with altered context on day 2 (b), and context testing and cued testing with altered context performed 1 month after the last conditioning (c) were determined. Distance traveled in each time window of the conditioning phase was determined (d). Activity suppression ratios were determined for the context test (e) and cued test (f). g-l Effects of Rapgef6 deficiency. The tests examining the effects of Rapgef6 deficiency were performed as in a-f, and percentages of freezing time (g-i), distance traveled in the conditioning phase (j), and activity suppression ratios (k, l) were determined