Fig. 3.
Assessing normalization strategies for miRNA relative quantification. Four candidate controls were used to normalize OpenArray results in two different combinations—one including U6 and one not, as well as U6 alone; and compared to the global normalization used for OpenArray analysis. a Each candidate control was assessed individually in terms of percentage detection and mean CRT of candidate controls across all samples and biological groups tested using the OpenArray system, These candidate controls were also assessed across the four normalization strategies in terms of b number of significant miRNA, and further to this c significant miRNA from global normalization that were also significant with other normalization strategies, and, of those significant and common miRNA, how many from the list of those chosen for further validation by RT-qPCR. d Venn Diagram shows the overlap between statistically significant miRNA as determined by all normalization methods